General Western Blot Reagents
Abcam PS48 (DMSO solution), PDK1 activator, 5MG
MW 286.8 Da, Purity >95%. Allosteric PDK1 activator. Stimulates PI3-kinase and phosphorylates AKT. Binds to the PIF pocket in the small lobe of PDK1. Shows developmental effects in vivo.
The product is subject to the following: Abcam Restricted Use Statement
Abcam N omega-Nitro-L-arginine methyl ester hydrochloride (L-NAME), NOS inhibitor, 100MG
MW 269.69 Da, Purity >98%. Cell-permeable NOS inhibitor. More soluble analog of arginine. (pIC₅₀ values are 4.3, 5.7 and 5.6 at human iNOS, nNOA and eNOS, respectively).
The product is subject to the following: Abcam Restricted Use Statement
Abcam Ibotenic acid (mM/ml), e x citoto x ic agonist, 10MM_ML
MW 158.11 Da, Purity >98%. Neuroexcitatory amino acid originally isolated from Amanita species. Neurotoxin often used to model cognitive dysfunctions. NMDA and metabotropic receptor agonist.
The product is subject to the following: Abcam Restricted Use Statement
Research Products International Corp Blot Stripping Buffer 4X Solution, 500 Milliliters
Product Formulation: TRIS-HCl: 250 mM; SDS: 8%; pH: 6.8. Add mercaptoethanol according to protocol.
Instructions :
1. Dilute 4X Stripping Bufffer ( 1part Stripping Buffer: 3 parts distilled water) and add beta-mercaptoethanol (0.6%).
2. Wash the wet membrane in the stripping buffer for 5 minutes.
3. Incubate the membrane in stripping buffer for 30 minutes at 65 degrees Celius while agitating.
LICOR WesternSure ECL Stripping Buffer for Chemiluminescent Westerns (100 mL)
WesternSure ECL Stripping Buffer is ideal for stripping and reprobing chemiluminescent Western blots. This Western blot stripping buffer. It removes primary and secondary antibodies from PVDF or nitrocellulose membranes, maintains target antigen integrity for efficient reprobing, and it does not require hazardous shipping, unlike many other stripping buffers. Stripping buffers are not recommended if bands need to be quantified. Note: WesternSure ECL Stripping Buffer is not recommended for loading amounts over 30 µg. It will demonstrate decreased performance when used at 2X or 5X concentration and heating the solution will not increase the stripping efficiency.
Biotang Inc Nitrocellulose Stripping Buffer(10X), 250 ml
Nitrocellulose Stripping Buffer(10X), 250 ml.
